Excessively High Event Rate During Acquisition

Symptom

Flow cytometer records excessive events per second (>10,000/sec), leading to coincidence errors where multiple cells pass through the laser simultaneously. Data shows abnormal event clustering and unreliable fluorescence measurements.

1. 1 Cell concentration too high (above 1x10^6 cells/mL), causing multiple cells to be analyzed simultaneously
2. 2 Sample overcorrection during preparation, providing excessive margin for error

1. 1 Dilute sample to optimal concentration between 1x10^5 and 1x10^6 cells/mL
2. 2 Re-count cells using hemocytometer or automated cell counter before preparing final sample; aim for mid-range concentration to balance event rate and acquisition time

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