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Wound Healing Assay moderate

Scratch is too wide or uneven

Symptom
Scratch edges are rough and the width varies along the line, making it impossible to accurately compare migration distances later.
Common Causes
  1. 1 Pressure on the pipette tip is uneven
  2. 2 Different scratch tools used across wells
  3. 3 Monolayer confluence is not uniform
Solutions
  1. 1 Use the same model of pipette tip — or better, a dedicated scratch tool
  2. 2 Move in a single straight line at constant speed
  3. 3 Plate evenly and confirm consistent monolayer before scratching
Related Video (3)
YouTube (Curated Tutorials) ★ 85
Scratch Assay Tutorial
"Directly teaches scratch assay technique and proper execution to avoid uneven scratches"
YouTube (Curated Tutorials) ★ 82
Wound healing
"Covers setup and execution of wound healing assay with focus on proper methodology"
YouTube (Curated Tutorials) ★ 72
Quantitative analysis of Wound Healing, Cell Migration or Scratch Assay using ImageJ Software
"Demonstrates quantitative analysis of scratch assay results, showing how uneven scratches affect measurement accuracy"
Source: xiaohongshu.com ↗
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