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Wound Healing Assay moderate

Scratch is too wide or uneven

Symptom
Scratch edges are rough and the width varies along the line, making it impossible to accurately compare migration distances later.
Common Causes
  1. 1 Pressure on the pipette tip is uneven
  2. 2 Different scratch tools used across wells
  3. 3 Monolayer confluence is not uniform
Solutions
  1. 1 Use the same model of pipette tip — or better, a dedicated scratch tool
  2. 2 Move in a single straight line at constant speed
  3. 3 Plate evenly and confirm consistent monolayer before scratching
Related Video (3)
YouTube (Curated Tutorials) ★ 90
Scratch Assay Tutorial
"Direct scratch assay tutorial showing correct pipette-tip technique to avoid uneven scratches."
YouTube (Curated Tutorials) ★ 85
Wound healing
"Demonstrates wound healing assay setup and analysis, directly addressing the affected technique."
YouTube (Curated Tutorials) ★ 60
Quantitative analysis of Wound Healing, Cell Migration or Scratch Assay using ImageJ Software
"Focuses on quantifying migration distances, useful context for why scratch width consistency matters."
Source: xiaohongshu.com ↗
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