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Western Blot (Sigma Protocol) minor

Non-parallel Bands

Symptom
Protein bands appear curved, wavy, or not parallel to each other
Common Causes
  1. 1 Gel has not polymerized evenly or parallel
Solutions
  1. 1 Review gel preparation procedure to ensure even polymerization
  2. 2 Ensure casting apparatus is level during gel polymerization
Related Video (2)
Cell Signaling Technology ★ 85
Western Blot Troubleshooting Guide
"Directly addresses Western blot troubleshooting with diagnostic guidance for common problems including gel-related issues"
Bilibili (China-Accessible Mirrors) ★ 78
Western blot hands-on: Protein extraction through detection
"Emphasizes troubleshooting common pitfalls in hands-on protocol, likely covering gel polymerization and band quality issues"
Source: sigmaaldrich.com ↗
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