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Western Blot (Sigma Protocol) minor

Non-parallel Bands

Symptom
Protein bands appear curved, wavy, or not parallel to each other
Common Causes
  1. 1 Gel has not polymerized evenly or parallel
Solutions
  1. 1 Review gel preparation procedure to ensure even polymerization
  2. 2 Ensure casting apparatus is level during gel polymerization
Related Video (3)
Cell Signaling Technology ★ 85
Western Blot Troubleshooting Guide
"Dedicated troubleshooting guide explicitly addresses Western blot problems and solutions, directly applicable to diagnosing non-parallel band artifacts."
Bilibili (China-Accessible Mirrors) ★ 78
Western blot hands-on: Protein extraction through detection
"Hands-on protocol with emphasis on troubleshooting common pitfalls provides practical context for preventing gel polymerization issues that cause band distortion."
Bio-Rad Laboratories ★ 72
Western Blotting
"Demonstrates SDS-PAGE gel preparation and protein separation fundamentals, showing proper gel setup to prevent polymerization irregularities."
Source: sigmaaldrich.com ↗
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