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Western Blot (Sigma Protocol) minor

Non-parallel Bands

Symptom
Protein bands appear curved, wavy, or not parallel to each other
Common Causes
  1. 1 Gel has not polymerized evenly or parallel
Solutions
  1. 1 Review gel preparation procedure to ensure even polymerization
  2. 2 Ensure casting apparatus is level during gel polymerization
Related Video (3)
Cell Signaling Technology ★ 90
Western Blot Troubleshooting Guide
"Troubleshooting guide that directly addresses diagnosing and fixing problems like non-parallel/curved bands, highly relevant to this failure case."
Bilibili (China-Accessible Mirrors) ★ 70
Western blot hands-on: Protein extraction through detection
"Hands-on protocol with explicit emphasis on troubleshooting common pitfalls, useful for understanding proper gel casting and electrophoretic conditions that prevent band distortion."
Bilibili (China-Accessible Mirrors) ★ 68
Reliable and Reproducible Western Blot Results
"CST webinar on reliable and reproducible WB results covers proper technique including gel polymerization and running conditions that cause band artifacts."
Source: sigmaaldrich.com ↗
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