Sample Out of Range - Too High (Nucleic Acid Quantification)

Question

Accepted Answer

Qubit Fluorometer screen indicates samples are OUT OF RANGE (too high), sample fluorescence exceeds highest standard Common causes: 1) Sample concentration exceeds assay detection range 2) Using high sensitivity (HS) assay for highly concentrated samples 3) Too much sample volume used in assay mixture Solutions: 1) Dilute sample and repeat measurement 2) Use smaller volume of sample in assay (less than standard volume) 3) Switch from HS assay to BR (Broad Range) assay for lower sensitivity 4) For DNA: switch from Qubit DNA HS to Qubit DNA BR Assay 5) For RNA: switch from Qubit RNA HS to Qubit RNA BR Assay