Home › Failure Case Library › Granular Precipitate on Cells
Lipid Transfection minor

Granular Precipitate on Cells

Symptom
Small granular precipitate visible microscopically on cells after adding transfection complexes
Common Causes
  1. 1 Excess EDTA present in DNA dilution buffer
  2. 2 Excess cationic lipid present during complex formation
  3. 3 EDTA concentration >0.3 mM in TE buffer
Solutions
  1. 1 Dilute DNA in water instead of TE buffer
  2. 2 If TE preferred, use EDTA concentrations <0.3 mM
  3. 3 Ensure cationic lipid concentrations do not exceed recommended amounts
  4. 4 Note: precipitate presence/absence is not indicative of transfection performance
Related Video (2)
YouTube (Curated Tutorials) ★ 78
Plasmid DNA Transfection Protocol
"Directly covers Lipofectamine LTX protocol with optimized steps to prevent transfection complex formation issues like precipitate"
Bilibili (China-Accessible Mirrors) ★ 72
Cell Transfection Operation: Common Mistakes Avoided
"Explicitly addresses common mistakes and troubleshooting in transfection, likely including buffer preparation errors that cause granular precipitate"
Source: thermofisher.com ↗
← Back to all cases