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Lipid Transfection severe

Cell Death After RNAiMAX Transfection

Symptom
Significant cell death observed following transfection with Lipofectamine RNAiMAX reagent
Common Causes
  1. 1 Antibiotics added to medium during transfection
  2. 2 Serum-free media incompatible with Lipofectamine RNAiMAX
Solutions
  1. 1 Do not add antibiotics to medium during transfection
  2. 2 Test serum-free media for compatibility with Lipofectamine RNAiMAX reagent before use
Related Video (3)
YouTube (Curated Tutorials) ★ 95
siRNA Transfection Protocol
"Directly demonstrates siRNA transfection with Lipofectamine RNAiMAX protocol, the exact reagent and technique involved in the failure case"
Bilibili (China-Accessible Mirrors) ★ 78
Cell Transfection Operation: Common Mistakes Avoided
"Focuses on common mistakes and troubleshooting in transfection protocols, directly applicable to identifying why antibiotic addition causes cell death"
Bilibili (China-Accessible Mirrors) ★ 72
siRNA Transfection Experimental Protocol
"Provides hands-on siRNA transfection protocol demonstration relevant to understanding proper execution and avoiding reagent-related toxicity issues"
Source: thermofisher.com ↗
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