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Percentage of reads mapping to the targeted sequence does not decrease after depletion treatment. Sequencing analysis shows target RNA remains at original levels.
Target RNA depletion efficiency is significantly reduced or absent. Sequencing shows minimal reduction in targeted reads despite proper protocol execution.
RNA depletion shows poor efficiency with high residual target reads. RNA quantification may be inaccurate and inconsistent with expected values.
Depletion efficiency is reduced or completely absent. Target RNA remains at high levels after treatment despite using validated probe designs.
Probe hybridization step was unsuccessful resulting in poor depletion. Target RNA removal is inconsistent or incomplete across replicate samples.
Depletion efficiency varies significantly across different targeted sequences. Some target regions show good depletion while others remain at high levels.
Target sequence used to design probes differs from the reference used to evaluate depletion. Depletion appears ineffective when analyzed against current genome annotations.
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