Spin down all vials before opening to ensure contents settle at the bottom. Use filter pipette tips throughout the procedure to minimize contamination during handling.
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2
Maintain aseptic technique for RNA work
Use proper aseptic techniques when working with RNA samples. Clean working surfaces with RNAzap or similar products and change gloves frequently to prevent bacterial and mold contamination from hands and dust particles.
▶ 00:17
3
Store samples and reagents properly
Keep all sample and reagent vials capped when not in use. Use RNA and DNA-free plastics, water, and solutions for all work.
▶ 00:37
4
Handle and preserve isolated RNA
Keep purified RNA on ice at all times and avoid freeze-thaw cycles. Store purified RNA long-term at minus 20 degrees or minus 70 degrees in nuclease-free water.
▶ 00:50
5
Mix reaction components efficiently
Use nuclease-free water for dilutions and gently pipette up and down to mix reaction components without vortexing. Keep all master mixes on ice to maintain optimal enzyme activity.
▶ 01:05
6
Add reagents and manage enzymes
Keep everything on ice and use immediately after mixing reagents. Return enzymes to the freezer promptly after use to ensure product stability.
▶ 01:23
7
Store final library samples correctly
Store finished NGS library samples in TE buffer using low-bind DNA tubes to ensure long-term stability and prevent sample degradation.