Matthias explains that hydroxymethylfurfural (HMF) is an organic compound produced from fructose dehydration, and that measuring HMF content determines honey freshness and whether honey has been heated or stored long-term.
▶ 00:08
2
Prepare honey sample for analysis
Weigh 2.5 grams of honey and dilute it with distilled water, then fill the solution to 10 ml total volume with additional distilled water.
▶ 00:51
3
Gather materials and instrument setup
Retrieve the HMF test strips, ecoFlex 20 reflectometer, barcode reference card, and manual from the test kit box. Switch on the instrument and scan the barcode to select the HMF measurement parameter.
▶ 01:20
4
Load test strip into instrument
Open the test strip box and remove one strip. Press the start button on the instrument, then dip the test strip into the diluted honey sample for 1-2 seconds.
▶ 02:07
5
Measure HMF concentration by reflectometry
Insert the test strip into the instrument's measuring chamber. After approximately 10 seconds, the instrument automatically measures the reaction zone and displays the HMF result on the screen.
▶ 02:42
6
Calculate final HMF concentration
Apply the dilution correction formula provided in the application notes to convert the instrument reading to the actual HMF concentration in the original honey sample.
▶ 02:57
💬 Comments coming soon
New protocols and pitfalls, in your inbox
A short email when we add notable lab videos and failure cases. No spam, unsubscribe anytime.