Steps

1 Design panel with OptiBuild reagents 00:04
2 Perform titration test on reagent vial 00:32
3 Transfer titration points to flow tubes 00:53
4 Run samples on flow cytometer 00:59
5 Analyze results and identify best concentration 01:04
6 Confirm performance with histogram or dot plot 01:13

Immunology BD Biosciences

BD OptiBuild™ Titration Video

Name: BD OptiBuild™ Titration Video
Uploaded: 2026-06-19
Duration: 1 min 26 s
Description: This video demonstrates how to perform a simple titration test to identify the best amount to be used in your panel when using BD OptiBuild reagents.

Protocol

Difficulty

intermediate

Steps

Design panel with OptiBuild reagents

Researchers design a cytometry panel for their specific cytometer configuration using BD OptiBuild reagents, which offer a wide array of available clones and dyes through semi-custom manufacturing to meet research deadlines.

▶ 00:04

Perform titration test on reagent vial

Conduct a titration test on the 50 microgram OptiBuild reagent vial, starting at 1 microgram and titrating down a minimum of six points to determine the optimal concentration.

▶ 00:32

Transfer titration points to flow tubes

Transfer each titration point to corresponding flow cytometry tubes containing the desired cells for analysis.

▶ 00:53

Run samples on flow cytometer

Run the prepared samples on the flow cytometer instrument and collect data from all titration points.

▶ 00:59

Analyze results and identify best concentration

Analyze the flow cytometry results to find the best concentration that meets the desired performance criteria for the study panel.

▶ 01:04

Confirm performance with histogram or dot plot

Verify the optimal concentration performance by generating and reviewing histograms or dot plots from the analysis.

▶ 01:13

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