IP Immunoprecipitation Experiment Protocol Steps

severe

High Background with Nonspecific Bands

Western blot shows high background signal with multiple nonspecific bands, obscuring the target protein band and making interpretation difficult.

💡 6 · ✓ 6

severe

Bead-Antibody Isotype Incompatibility

No or very weak target protein signal despite using adequate antibody concentration, suggesting inefficient antibody capture by the beads due to isotype incompatibility.

💡 5 · ✓ 5