R&D Quantikine ELISA Protocol Video Tutorial

critical

Absent Standard Curve Signal with Normal Zero Standard

No detectable signal is observed for standard curve points containing analyte, while the zero standard (maximum binding control) shows normal OD values, indicating complete loss of competitive inhibition.

💡 4 · ✓ 4

moderate

High Variability Between Experimental Runs

Standard curves or sample values differ significantly between experiments run on different days, despite using the same reagents and protocol, showing poor reproducibility.

💡 5 · ✓ 5

moderate

B/B0 Ratio at Standard Curve Endpoint Out of Range

The B/B0 ratio (bound/maximum bound) at the lowest or highest standard concentration is either >95% or <5%, indicating the standard curve dynamic range is not properly positioned.

💡 3 · ✓ 3