Flow Cytometry QC Operation

severe

Compensation beads saturate detectors causing overflow

Compensation bead populations appear off-scale or saturate detectors, producing signals outside the linear detection range and preventing accurate compensation matrix calculation.

💡 4 · ✓ 4

severe

Tandem dye degradation on compensation beads

Tandem fluorophore compensation beads show spectral shift or altered emission profile over time, causing incorrect spillover calculation and poor compensation in acceptor channels.

💡 4 · ✓ 5

severe

Poor spectral reference controls for unmixing algorithms

Spectral unmixing (Cytek Aurora, Sony ID7000) produces residual spillover or negative populations despite using compensation beads, indicating unreliable full-spectrum reference signatures.

💡 5 · ✓ 5