Plasmid DNA Extraction Miniprep Protocol

critical

No DNA Recovered from Plasmid Miniprep

No detectable plasmid DNA is recovered after miniprep purification. Column elution yields no nucleic acid by spectrophotometry or gel electrophoresis.

💡 4 · ✓ 4

severe

Low Plasmid Yield Due to Incomplete Lysis

Plasmid miniprep yields are significantly below expected levels. A260/280 ratio may be normal but total DNA concentration is low.

💡 5 · ✓ 5

severe

Mini-prep yields almost no plasmid DNA

Eluate DNA concentration is very low; diagnostic digest and gel show no detectable target band.

💡 4 · ✓ 4

moderate

Low DNA Performance in Downstream Applications Due to Contaminants

Purified DNA fails or performs poorly in downstream applications (transformation, restriction digestion, PCR, sequencing) despite acceptable concentration and A260/280 ratio.

💡 6 · ✓ 6