Trizol RNA Extraction: Protocol & Principles

severe

Poor RNA Performance in Downstream Applications

Purified RNA fails to perform as expected in RT-PCR, RT-qPCR, RNA-seq library preparation, or other downstream assays despite acceptable concentration and A260/280 ratio.

💡 4 · ✓ 5

severe

Low RNA Yield After Cleanup

Recovered RNA concentration is significantly lower than expected based on input amount. Spectrophotometric measurement shows inadequate RNA quantity for downstream applications.

💡 5 · ✓ 5

moderate

Low A260/230 Ratio Indicates Contaminant Carryover

Spectrophotometric analysis shows A260/230 ratio below 1.8-2.0, indicating residual guanidine salt or other chaotropic agent contamination in the purified RNA.

💡 4 · ✓ 5