Co-IP Immunoprecipitation: Detailed Steps and Key Analysis

severe

Bead-Antibody Isotype Incompatibility

No or very weak target protein signal despite using adequate antibody concentration, suggesting inefficient antibody capture by the beads due to isotype incompatibility.

💡 5 · ✓ 5

severe

Protein Complex Disruption in Co-IP

In co-immunoprecipitation experiments, expected interacting proteins are not detected on Western blot, suggesting protein-protein complexes were disrupted during sample handling.

💡 3 · ✓ 3