Co-IP Immunoprecipitation: Detailed Steps and Key Analysis

severe

Protein Complex Disruption in Co-IP

In co-immunoprecipitation experiments, expected interacting proteins are not detected on Western blot, suggesting protein-protein complexes were disrupted during sample handling.

💡 3 · ✓ 3

moderate

Accidental Bead Loss During Aspiration

Inconsistent or absent target protein signal across replicate samples, with visible reduction in bead pellet volume, indicating beads were aspirated during wash or supernatant removal steps.

💡 3 · ✓ 4