Harvesting patient-derived xenografts for serial transplantation

Overview of the patient-derived xenograft harvesting procedure and examination of the euthanized animal with a large breast cancer tumor graft in the mammary fat pad.

Spray the tumor area with 70% ethanol to maintain sterility during the dissection procedure.

Use sterile forceps and scissors to cut away the external skin surrounding the tumor to reveal the bulk of the tumor tissue.

Use additional sterile utensils to carefully dissect and isolate the internal tumor from surrounding tissue.

Select downstream processing options including cryopreservation, freezing, formalin fixation, or sectioning tumor chunks for direct transplantation into recipient animals.