Home Cell Biology Gastrocnemius dissection.
Steps
  1. 1 Retract skin to expose muscle --:--
  2. 2 Pin foot and remove fat pad 00:25
  3. 3 Separate Achilles tendon from muscle 00:48
  4. 4 Cut Achilles tendon at ankle 01:08
  5. 5 Remove soleus muscle if present 01:18
  6. 6 Detach gastrocnemius at origin 01:35
Cell Biology Bio-protocol Video Citable · DOI

Gastrocnemius dissection.

DOI: 10.21769/BioProtoc.v118
Protocol
Difficulty
intermediate

Steps

1
Retract skin to expose muscle

Use hemostats to retract the skin from the ankle up to the hip, exposing the underlying gastrocnemius muscle and Achilles tendon.

▶ --:--
2
Pin foot and remove fat pad

Pin the foot in place using a pin, then carefully remove the fat pad overlying the gastrocnemius by snipping underneath and at the sides.

▶ 00:25
3
Separate Achilles tendon from muscle

Insert scissors beneath the Achilles tendon and open them to stretch and separate the tendon from the underlying muscle body.

▶ 00:48
4
Cut Achilles tendon at ankle

Snip the Achilles tendon at its distal end near the ankle to free the gastrocnemius from the lower leg.

▶ 01:08
5
Remove soleus muscle if present

Lift the gastrocnemius and check for the soleus muscle adhering to it. If present, carefully remove it to isolate the gastrocnemius.

▶ 01:18
6
Detach gastrocnemius at origin

Snip the gastrocnemius perpendicular to the bone at its proximal point of origin to completely free the muscle.

▶ 01:35
💬 Comments coming soon