Home › Cell Biology › Subcutaneous transplantation of tumorgrafts
Steps
  1. 1 Prepare tumor fragments in PBS solution --:--
  2. 2 Anesthetize animal with isoflurane 00:25
  3. 3 Prepare surgical site on dorsal flank 00:35
  4. 4 Administer analgesic and create incision 00:44
  5. 5 Create subcutaneous pocket and implant tumor 01:35
  6. 6 Close surgical site with sterile clips 01:55
  7. 7 Monitor animal recovery on warming platform 02:13
Cell Biology Bio-protocol Video Citable · DOI

Subcutaneous transplantation of tumorgrafts

DOI: 10.21769/BioProtoc.v12
Protocol
Difficulty
intermediate

Steps

1
Prepare tumor fragments in PBS solution

Thawed cryopreserved tumor fragments are placed in PBS solution with penicillin-streptomycin to maintain viability. The viable tumor pieces are ready for transplantation.

▶ --:--
2
Anesthetize animal with isoflurane

The animal is anesthetized in an anesthesia chamber using isoflurane gas and then maintained under anesthesia via a nose cone delivering continuous isoflurane.

▶ 00:25
3
Prepare surgical site on dorsal flank

The dorsal flank region is shaved with clippers to remove hair, then cleaned sequentially with povidone iodine swab followed by sterile alcohol pad for asepsis.

▶ 00:35
4
Administer analgesic and create incision

Ketoprofen is administered as a preoperative analgesic. Forceps are used to elevate the skin and a small incision is made in the prepared surgical area.

▶ 00:44
5
Create subcutaneous pocket and implant tumor

A subcutaneous pocket is created in the tissue beneath the incision. The tumor fragment is carefully placed into the pocket at least one centimeter away from the incision site.

▶ 01:35
6
Close surgical site with sterile clips

The incision is closed using sterile wound clips to secure the skin edges together at the surgical site.

▶ 01:55
7
Monitor animal recovery on warming platform

The animal is placed in a cage on a warming platform and observed until it regains normal activity and full consciousness.

▶ 02:13
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