Home › Biochemistry › Assembly of the transfer pack in a semi-dry blotter
Steps
  1. 1 Prepare materials for transfer pack assembly --:--
  2. 2 Arrange gel and membrane stack 00:28
  3. 3 Add buffer-saturated filter papers 00:43
  4. 4 Complete transfer pack assembly 00:57
Biochemistry Bio-protocol Video Citable · DOI

Assembly of the transfer pack in a semi-dry blotter

DOI: 10.21769/BioProtoc.v225
Protocol
Difficulty
intermediate

Steps

1
Prepare materials for transfer pack assembly

Gather and organize all necessary components for assembling the transfer pack. Ensure all materials are ready and properly positioned for the semi-dry blotter setup.

▶ --:--
2
Arrange gel and membrane stack

Layer the gel and membrane components in the correct sequence. Position each layer carefully to ensure proper alignment for protein transfer.

▶ 00:28
3
Add buffer-saturated filter papers

Place buffer-saturated filter papers on top of the membrane stack. These papers facilitate even distribution of electrical current during transfer.

▶ 00:43
4
Complete transfer pack assembly

Finalize the transfer pack by securing all components together. Verify that the assembly is complete and ready for installation in the blotter apparatus.

▶ 00:57
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