Flux vs. time

The video introduces the flux-versus-time R script used to estimate dose and time-dependent responses based on red to green fluorescent protein ratios. This script will analyze experimental data and generate visualizations of the results.

Create a two-column tab-delimited text file in Excel with treatment names and start times. The file must be named info.txt and saved in the correct format, as the data must match the experimental file names exactly.

Use File > Save As to save the Excel spreadsheet as a tab-delimited .txt file named info. Ensure proper formatting to avoid auto-formatting issues by converting to text format if needed.

In RStudio, select the fluxvstime.r script from the R scripts folder and use Code > Source to load the file. This will initiate the analysis workflow.

When prompted by the script, enter the number of time points from the experiment. In this example, three time points were scanned, so enter 3 and press Enter.

The script generates a summary graph showing normalized fluorescent protein ratios over time in hours, normalized to the vehicle control. The output includes both overall and per-seedling data summaries.

Review the organized data in the generated summary file. Perform appropriate statistical tests using R or other software like Origin, JMP, or GraphPad Prism based on your specific experimental design.