Home Microbiology COVID-19 Nucleic Acid Detection
Microbiology Bio-protocol Video Citable · DOI

COVID-19 Nucleic Acid Detection

DOI: 10.21769/BioProtoc.v288

🚨 Failure Case Library (2) + Submit your own case

critical
No-Template Control Turns Yellow (Contamination)
The no-template control (NTC) reaction turns yellow after incubation at 65°C, indicating contamination with amplifiable target nucleic acid or positive control template.
💡 4 · ✓ 6
severe
Internal Control Fails Due to Sample pH Incompatibility
The internal control reaction fails to turn yellow after incubation at 65°C when spiked into sample, though it works in water, indicating sample matrix interference.
💡 4 · ✓ 5
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