Home › Microscopy & Imaging › Preparation of the Lab-Tek® observation chamber for FM4-64 staining.
Steps
  1. 1 Prepare Lab-Tek chamber for staining --:--
  2. 2 Apply FM4-64 stain to specimen 00:35
  3. 3 Complete staining and prepare for imaging 01:00
Microscopy & Imaging Bio-protocol Video Citable · DOI

Preparation of the Lab-Tek® observation chamber for FM4-64 staining.

DOI: 10.21769/BioProtoc.v38
Protocol
Difficulty
intermediate

Steps

1
Prepare Lab-Tek chamber for staining

Set up the Lab-Tek observation chamber and gather materials needed for FM4-64 dye staining protocol.

▶ --:--
2
Apply FM4-64 stain to specimen

Add FM4-64 dye solution to the prepared chamber containing the biological sample.

▶ 00:35
3
Complete staining and prepare for imaging

Allow FM4-64 stain to incubate and finalize chamber preparation for fluorescence microscopy observation.

▶ 01:00
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