Experimental set-up for in vivo NMR measurements

The instructor presents the three main components needed: a wooden skewer with e-chrome wire, an NMR tube with attached string, and a glass capillary reference solution. The NMR tube assembly structure is then detailed, including the external tube, internal tube, cork with Teflon tubings for solution delivery and withdrawal.

Fungal material is carefully removed from storage flasks and placed gently into the bottom of the external NMR tube using a wooden skewer. Multiple fungal samples are added sequentially to the tube.

A sample depth gauge is used to check that sufficient fungal material has been added to the NMR tube.

The glass capillary containing reference solution is carefully placed into the NMR tube and pushed to the bottom using the wooden skewer's flat end.

The internal NMR tube is screwed onto the external tube. If the reference capillary obstructs this process, it is repositioned to the middle of the tube using the skewer's hook before completing assembly with yellow silicone film seal.

The three Teflon tubings are inserted into the NMR tube and their height is fixed by adjusting the black screw cap to ensure proper positioning.

Pure oxygen is bubbled through the solution to aerate it, and the solution flow rate is adjusted while monitoring the solution level in the NMR tube to ensure proper delivery to the sample.