Home › Neuroscience › Trace Fear Conditioning Protocol.
Steps
  1. 1 Overview of trace fear conditioning protocol --:--
  2. 2 Configure freeze frame software and shock parameters 00:53
  3. 3 Prepare chamber and conduct conditioning trial 02:08
  4. 4 Post-trial chamber cleaning and mouse removal 03:03
  5. 5 Perform 24-hour contextual memory test 03:29
  6. 6 Prepare dark chamber for tone-trace test 04:20
  7. 7 Execute tone and trace protocol test 04:37
Neuroscience Bio-protocol Video Citable · DOI

Trace Fear Conditioning Protocol.

DOI: 10.21769/BioProtoc.v72
Protocol
Difficulty
intermediate

Steps

1
Overview of trace fear conditioning protocol

Introduction to the hippocampal performance task with timeline overview. Describes conditioning day procedures, 24-hour context testing, and 48-hour tone-trace testing.

▶ --:--
2
Configure freeze frame software and shock parameters

Open freeze frame software, select trace fear conditioning protocol, and configure shock intensity to 0.5 mA. Set up the equipment with proper meter range and routing settings.

▶ 00:53
3
Prepare chamber and conduct conditioning trial

Clean the conditioning chamber with 10% ethanol, place mouse in chamber, enter subject details, and run the conditioning protocol with five tone-trace-shock trials.

▶ 02:08
4
Post-trial chamber cleaning and mouse removal

Remove mouse from conditioning chamber after trial completion and clean the chamber thoroughly with 10% ethanol between successive animals.

▶ 03:03
5
Perform 24-hour contextual memory test

Conduct context protocol testing 24 hours after conditioning. Place mouse in chamber for 300 seconds without tone or shock stimuli.

▶ 03:29
6
Prepare dark chamber for tone-trace test

Set up testing 48 hours after conditioning using dark chambers with black plastic covering the grid floor to create a new context. Set up infrared camera for recording.

▶ 04:20
7
Execute tone and trace protocol test

Select tone and trace protocol, place mouse in dark chamber, record animal behavior with infrared camera during the test, then remove mouse and clean chamber with water.

▶ 04:37
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