Home › Cell Biology › Gelatinization of glass-bottom dishes.
Steps
  1. 1 Introduction and materials overview 00:01
  2. 2 Clean imaging disc with PPS 00:12
  3. 3 Prepare and add gelatin solution 00:54
  4. 4 Distribute gelatin evenly across plate 01:11
  5. 5 Incubate gelatin-coated dish overnight 01:27
Cell Biology Bio-protocol Video Citable · DOI

Gelatinization of glass-bottom dishes.

DOI: 10.21769/BioProtoc.v82
Protocol
Difficulty
intermediate

Steps

1
Introduction and materials overview

The presenter introduces themselves and explains the purpose of the protocol. They identify the materials needed for gelatinizing imaging dishes.

▶ 00:01
2
Clean imaging disc with PPS

Add approximately 2 ml of PPS (propylene glycol propyl ether) to the imaging disc and then remove it completely. This cleaning step prepares the surface for gelatin coating.

▶ 00:12
3
Prepare and add gelatin solution

Add 2 to 3 ml of 0.2% gelatin solution in milli-Q water to the cleaned imaging disc. The gelatin should be autoclaved prior to use for sterilization.

▶ 00:54
4
Distribute gelatin evenly across plate

Ensure the gelatin spreads uniformly across the entire surface of the imaging dish to achieve consistent coating.

▶ 01:11
5
Incubate gelatin-coated dish overnight

Place the gelatinized imaging dish in a 37°C incubator overnight to allow the gelatin to set and ensure proper coating.

▶ 01:27
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