Home › Microscopy & Imaging › Replacing ES cell medium with live-cell imaging medium
Steps
  1. 1 Remove the culturing medium 00:02
  2. 2 Add imaging medium to the dish 00:40
  3. 3 Prepare dish for transport to microscope 01:47
  4. 4 Begin live-cell imaging 02:20
Microscopy & Imaging Bio-protocol Video Citable · DOI

Replacing ES cell medium with live-cell imaging medium

DOI: 10.21769/BioProtoc.v85
Protocol
Difficulty
intermediate

Steps

1
Remove the culturing medium

After the 30-minute incubation period, remove the existing culturing medium from the imaging dish. This prepares the cells for the switch to live-cell imaging medium.

▶ 00:02
2
Add imaging medium to the dish

Add approximately 2 ml of phenol red-free emitting medium to the imaging disk. This specialized medium is required for proper live-cell imaging without fluorescence interference.

▶ 00:40
3
Prepare dish for transport to microscope

Prepare the imaging dish for transport by placing it in a protective holder with a p100 petri dish as the base and a p60 dish as the top. Cover the top with electrical tape to block ambient light during transport.

▶ 01:47
4
Begin live-cell imaging

The sample is now ready for imaging on the microscope. Proceed to the imaging station and begin live-cell imaging.

▶ 02:20
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