Brain removal, dissection and homogenization

Set up all materials on ice and perform a mid-line incision from posterior to anterior along the scalp to expose the skull.

Make careful cuts through the lateral cranial structures from neck to nose, and perform additional cuts toward the occipital region to gain full access to the brain.

Carefully remove the whole brain from the skull using a small spatula.

Use fine dissection forceps to remove the cerebellum, brainstem, and white matter to isolate clean brain cortices.

Perform a quick rinse of the dissected brain tissue in non-sterile PBS to remove debris.

Use a potter with pestle and glass tube to homogenize the fresh brain tissue into a uniform suspension.