Collection and Placement of NEDAS Organoids for Sectioning in OCT Molds

Set up the laboratory workspace and gather all necessary materials for NEDAS organoid sectioning, including OCT molds and collection containers.

Carefully collect the NEDAS organoids from their culture vessel using appropriate pipetting techniques to maintain organoid integrity.

Pipette the collected NEDAS organoids into the OCT (optimal cutting temperature) mold, positioning them appropriately for sectioning.

Adjust the position and orientation of organoids within the OCT mold to ensure optimal alignment for downstream sectioning and analysis.