Expert tips for mastering chromatography column tag removal

Learn how protein tags enable simple one-step purification, minimizing complexity and speeding up access to pure proteins for research purposes.

Discover how ÄKTA chromatography systems automate on-column tag cleavage, allowing researchers to focus on meaningful scientific work rather than manual purification.

Recognize that GST tags increase protein solubility but may interfere with function, requiring protease digestion and on-column cleavage to remove the tag while preserving native protein structure.

Bind GST-tagged protein to a GS Trap Fast Flow column and automatically cleave the tag using precision protease, then pass the product through a second GS Trap column to separate the target protein from protease and free GST.

Use a fusion protein with a thrombin cleavage site between the protein and GST tag, then automate cleavage with thrombin and remove the protease using a High Trap Benzamidine column.

Explore additional on-column cleavage options such as recombinant Factor Xa for GST tags and automated cleavage with active protease for histidine tags when tag removal is necessary.