Mix approximately 30 grams of microbial mat thoroughly using a sterile grinding pestle without grinding until no lumps remain visible in the mixture.
Separate microbial cells from the homogenized mat background matrix using the prepared sample.
Apply freeze-thaw cycles combined with chemical lysis methodology to break open the microbial cells and release DNA.
Perform organic extractions and RNase treatment to eliminate cell debris and RNA from the lysate, leaving DNA in solution.
Isolate DNA from the treated solution using extraction methodology to separate it from other contaminants.
Perform DNA precipitation and purification steps to obtain high molecular weight DNA of optimal quality and concentration.
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