Home › Genetics / Genomics › High Efficiency CRISPR Gene Editing with Bio-Rad's Gene Pulser Xcell Electroporation System
Steps
  1. 1 Understand CRISPR biological mechanism --:--
  2. 2 Learn CRISPR gene editing principles 00:56
  3. 3 Identify challenges in CRISPR transfection 01:53
  4. 4 Select appropriate electroporation system 02:15
Genetics / Genomics Bio-Rad Laboratories

High Efficiency CRISPR Gene Editing with Bio-Rad's Gene Pulser Xcell Electroporation System

Protocol
Difficulty
intermediate

Steps

1
Understand CRISPR biological mechanism

Learn how bacteria naturally use CRISPR as an immune defense system against bacteriophages by storing viral DNA sequences and comparing them to future invaders. Understand how this natural mechanism can be repurposed for genome engineering applications.

▶ --:--
2
Learn CRISPR gene editing principles

Discover how scientists exploit the CRISPR system by introducing pre-designed RNA strands and Cas9 protein to target and activate or deactivate specific genes in cells. Recognize the applications of CRISPR in treating disease and modifying organisms.

▶ 00:56
3
Identify challenges in CRISPR transfection

Acknowledge the difficulty of achieving high transfection efficiency and reproducible results in CRISPR experiments, which are critical for successful genome engineering outcomes.

▶ 01:53
4
Select appropriate electroporation system

Choose Bio-Rad's Gene Pulser Xcell electroporation system as the solution for efficient delivery of nucleic acids and proteins to cells. Recognize its preset protocols for common cell types and comprehensive online protocol library.

▶ 02:15
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