How can I tell if I sequenced through the insert? Part 1 | Illumina Video

moderate

Residual Adaptor or Primers After PCR

Adaptor or primer peaks visible on Bioanalyzer or similar instrument after PCR amplification, indicating inefficient cleanup or excess reagent usage.

💡 3 · ✓ 3

moderate

Library Not the Correct Insert Size

Library fragment size distribution is shifted from expected range, with either smaller or larger inserts than intended, or discrepancy between fragment analyzer measurement and sequencer-determined insert size.

💡 5 · ✓ 5