Home Cell Biology How Can Sso7d Fusion Technology Help Your PCR?
Steps
  1. 1 Identify limitations of standard PCR reagents --:--
  2. 2 Understand Sso7d protein binding mechanism 00:44
  3. 3 Recognize improved polymerase processivity benefits 01:21
  4. 4 Apply Sso7d technology to eliminate optimization challenges 01:39
Cell Biology Bio-Rad Laboratories

How Can Sso7d Fusion Technology Help Your PCR?

Protocol
Difficulty
intermediate

Steps

1
Identify limitations of standard PCR reagents

The video introduces how conventional PCR reagents perform well for routine applications but fail with challenging samples containing high GC content, low abundance targets, or PCR inhibitors like blood.

▶ --:--
2
Understand Sso7d protein binding mechanism

The Sso7d fusion polymerase technology is explained as having greater affinity for double-stranded DNA compared to traditional polymerases, which allows it to stabilize the polymerase-template complex during replication.

▶ 00:44
3
Recognize improved polymerase processivity benefits

The enhanced ability of Sso7d fusion polymerase to travel reliably down DNA strands results in increased qPCR efficiency and higher sensitivity compared to standard polymerases.

▶ 01:21
4
Apply Sso7d technology to eliminate optimization challenges

Researchers can now confidently work with difficult assays, low-abundance targets, and PCR-inhibitor-contaminated samples without extensive troubleshooting using Sso7d super mixes.

▶ 01:39
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