4Apply Sso7d technology to eliminate optimization challenges01:39
Cell BiologyBio-Rad Laboratories
How Can Sso7d Fusion Technology Help Your PCR?
Protocol
Difficulty
intermediate
Steps
1
Identify limitations of standard PCR reagents
The video introduces how conventional PCR reagents perform well for routine applications but fail with challenging samples containing high GC content, low abundance targets, or PCR inhibitors like blood.
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2
Understand Sso7d protein binding mechanism
The Sso7d fusion polymerase technology is explained as having greater affinity for double-stranded DNA compared to traditional polymerases, which allows it to stabilize the polymerase-template complex during replication.
The enhanced ability of Sso7d fusion polymerase to travel reliably down DNA strands results in increased qPCR efficiency and higher sensitivity compared to standard polymerases.
▶ 01:21
4
Apply Sso7d technology to eliminate optimization challenges
Researchers can now confidently work with difficult assays, low-abundance targets, and PCR-inhibitor-contaminated samples without extensive troubleshooting using Sso7d super mixes.
▶ 01:39
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