Home › Analytical Chem › How to connect HiTrap Fibro PrismA unit to an ÄKTA system
Steps
  1. 1 Review HiTrap Fibro PrismA unit specifications 00:15
  2. 2 Disconnect flow restrictor and prepare unit 00:40
  3. 3 Wash out storage solution with low flow 01:02
  4. 4 Increase flow rate stepwise with leak checks 01:20
  5. 5 Establish recommended operating flow rate 01:33
  6. 6 Record baseline pressure reference values 01:43
Analytical Chem Cytiva

How to connect HiTrap Fibro PrismA unit to an ÄKTA system

Protocol
Difficulty
intermediate

Steps

1
Review HiTrap Fibro PrismA unit specifications

Understand that HiTrap Fibro PrismA units are ready-to-use systems designed for rapid monoclonal antibody capture and compatible with ÄKTA chromatography systems. Note that no drop-to-drop connection is required as air will be automatically flushed during high-flow operation.

▶ 00:15
2
Disconnect flow restrictor and prepare unit

Manually disconnect the flow restrictor from the unit to minimize back pressure, or use the pH valve if available. This step is essential before connecting to the ÄKTA system.

▶ 00:40
3
Wash out storage solution with low flow

Run the system at low flow rate to wash out the 20% ethanol storage solution from the unit. Air removal is automatic during this washing process.

▶ 01:02
4
Increase flow rate stepwise with leak checks

Gradually increase the flow rate using a stepwise approach, checking for leakage at each increment before proceeding to higher flow rates. Stop and investigate any leaks before continuing.

▶ 01:20
5
Establish recommended operating flow rate

Set the system to the recommended operating flow rate of 16 milliliters per minute for HiTrap Fibro PrismA operation.

▶ 01:33
6
Record baseline pressure reference values

Check and record the pre-column and delta-column pressure values as reference measurements before starting your chromatography run.

▶ 01:43
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