Home Immunology How to Isolate Cells in 3 Easy Steps using MACS MicroBeads
Steps
  1. 1 Magnetically label cells with MACS MicroBeads 00:08
  2. 2 Apply cell suspension to MACS column 00:40
  3. 3 Wash and separate labeled cells 01:10
  4. 4 Elute isolated cells from column 01:26
Immunology Miltenyi Biotec

How to Isolate Cells in 3 Easy Steps using MACS MicroBeads

Protocol
Difficulty
intermediate

Steps

1
Magnetically label cells with MACS MicroBeads

Add MACS MicroBeads to your cell suspension and incubate at 4°C for a few minutes. The nanoized beads are non-toxic and biocompatible, allowing cells to retain full functionality.

▶ 00:08
2
Apply cell suspension to MACS column

Place the MACS column in the magnetic field of a MACS separator and apply the labeled cell suspension. Unlabeled cells pass through while magnetically labeled cells are retained in the column.

▶ 00:40
3
Wash and separate labeled cells

The column matrix amplifies the magnetic field, allowing thorough washing of labeled cells without compromising yield. The column design maintains optimal spacing for cell movement.

▶ 01:10
4
Elute isolated cells from column

Remove the column from the magnetic separator and gently elute the labeled cells. The cells are fully functional and immediately ready for downstream applications including flow cytometry.

▶ 01:26
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