Home›Immunology›How to Isolate Cells in 3 Easy Steps using MACS MicroBeads
Steps
1Magnetically label cells with MACS MicroBeads00:08
2Apply cell suspension to MACS column00:40
3Wash and separate labeled cells01:10
4Elute isolated cells from column01:26
ImmunologyMiltenyi Biotec
How to Isolate Cells in 3 Easy Steps using MACS MicroBeads
Protocol
Difficulty
intermediate
Steps
1
Magnetically label cells with MACS MicroBeads
Add MACS MicroBeads to your cell suspension and incubate at 4°C for a few minutes. The nanoized beads are non-toxic and biocompatible, allowing cells to retain full functionality.
▶ 00:08
2
Apply cell suspension to MACS column
Place the MACS column in the magnetic field of a MACS separator and apply the labeled cell suspension. Unlabeled cells pass through while magnetically labeled cells are retained in the column.
▶ 00:40
3
Wash and separate labeled cells
The column matrix amplifies the magnetic field, allowing thorough washing of labeled cells without compromising yield. The column design maintains optimal spacing for cell movement.
▶ 01:10
4
Elute isolated cells from column
Remove the column from the magnetic separator and gently elute the labeled cells. The cells are fully functional and immediately ready for downstream applications including flow cytometry.
▶ 01:26
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