How to Isolate Treg Cells - Video Tutorial

Add Max Express buffer and Max Express T-Rex isolation cocktail to the whole blood sample. This cocktail labels non-CD4 positive cells with Max Express beads and CD25 positive cells with microbeads.

Place the sample in a Max mix tube rotator and incubate for 10 minutes using an overhead rotator. This ensures proper labeling of target and non-target cells throughout the sample.

Place the tube in the magnetic field of the Max Express separator for 15 minutes. Bead-labeled cells are drawn to the tube side while CD25 positive cells remain in the supernatant due to their small microbead size.

Transfer the supernatant containing CD4 positive cells onto a magnetic column for further isolation of the target T-Reg cells.

Wash the magnetic column twice, then elute the CD25 positive T-Reg cells. The purified T-Reg cells are now ready for downstream analysis.