Home › Biochemistry › How to set up a photometric assay for protein quantitation
Steps
  1. 1 Select protein type for assay 00:12
  2. 2 Select sample format 00:18
  3. 3 Define wells and blank controls 00:23
  4. 4 Start the photometric assay 00:32
  5. 5 Review protein concentration results 00:38
  6. 6 Choose absorbance value type 00:41
  7. 7 Export results to data software 00:46
Biochemistry Thermo Fisher Scientific

How to set up a photometric assay for protein quantitation

Protocol
Difficulty
intermediate

Steps

1
Select protein type for assay

Choose the appropriate protein type from the Thermo Scientific Multiskan Sky Microplate Spectrophotometer interface to configure the assay parameters.

▶ 00:12
2
Select sample format

Specify the sample format (microplate wells) for the spectrophotometer to properly detect and read your protein samples.

▶ 00:18
3
Define wells and blank controls

Select which wells contain samples and specify the number of blank controls needed for accurate baseline absorbance measurements.

▶ 00:23
4
Start the photometric assay

Initiate the assay run on the microplate reader to measure absorbance values across all selected wells.

▶ 00:32
5
Review protein concentration results

View the calculated IgG protein concentration values displayed on the spectrophotometer screen after assay completion.

▶ 00:38
6
Choose absorbance value type

Select between 'Blanked' (background-corrected) or 'Measured' (raw) absorbance values depending on your analysis needs.

▶ 00:41
7
Export results to data software

Save and export the assay results to Excel or the SkanIt software platform for further analysis and documentation.

▶ 00:46
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