Home › Cell Biology › Isolate RNA with the PureLink™ RNA Mini Kit (qPCR step 2)
Steps
  1. 1 Unbox kit and identify components --:--
  2. 2 Add lysed sample to collection tube 01:25
  3. 3 Centrifuge and wash column multiple times 01:53
  4. 4 Transfer spin column to recovery tube 02:23
  5. 5 Elute RNA with water and centrifuge 02:32
  6. 6 Collect final RNA sample 02:48
Cell Biology Thermo Fisher Scientific

Isolate RNA with the PureLink™ RNA Mini Kit (qPCR step 2)

Protocol
Difficulty
intermediate

Steps

1
Unbox kit and identify components

Open the PureLink RNA Mini Kit and lay out all components including lysis buffer, wash buffers 1 and 2, RNA-free water, recovery tubes, collection tubes, and spin cartridges.

▶ --:--
2
Add lysed sample to collection tube

Transfer the lysed and homogenized cell or tissue sample into a collection tube containing a spin cartridge, where RNA will bind to the column matrix.

▶ 01:25
3
Centrifuge and wash column multiple times

Centrifuge the collection tube to pass the sample through the spin column, then repeat the washing process three times using wash buffers 1 and 2 to remove contaminants.

▶ 01:53
4
Transfer spin column to recovery tube

Remove the spin column from the original collection tube and insert it into a fresh recovery tube to prepare for RNA elution.

▶ 02:23
5
Elute RNA with water and centrifuge

Add RNA-free water to the top of the spin column and centrifuge to elute the purified RNA into the recovery tube below.

▶ 02:32
6
Collect final RNA sample

Remove the spin column, close and label the recovery tube containing the purified RNA in water. The isolation is complete.

▶ 02:48
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