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Cell Biology JoVE (Open Access) Citable · DOI

3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue

DOI: 10.3791/55943-v
What you'll learn
  • ✓ Position subject for 3D ultrasound scanning
  • ✓ Reconstruct voxel array from raw data
  • ✓ Measure muscle volume and fascicle length
  • ✓ Validate measurements against cadaver data
Protocol

Biopharma Insights 3D ultrasound imaging (3DUS) allows fast and cost-effective morphometry of musculoskeletal tissues. We present a protocol to measure muscle volume and fascicle length using 3DUS.

Difficulty
intermediate
Total time
~30 min/sample
Model organism
Human cadaver

Steps

1
Position subject and locate landmarks

Position the gastrocnemius medialis subject and identify body landmarks to define the region of interest for imaging.

▶ 01:13
2
Acquire 3D ultrasound and reconstruct voxel array

Perform 3D ultrasound examination and reconstruct the voxel array from the acquired images.

▶ 02:43
3
Measure muscle morphology variables

Measure muscle volume and fascicle length from the reconstructed 3D ultrasound data.

▶ 04:32
4
Compare with cadaver validation data

Review representative cadaver validation results to confirm accuracy of the ultrasound measurements.

▶ 06:55
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