Home › Cell Biology › A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
Cell Biology JoVE (Open Access) Citable · DOI

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

DOI: 10.3791/57301-v
What you'll learn
  • ✓ Perform intra-prostatic injection of syngeneic prostate cancer cells in mice
  • ✓ Establish orthotopic tumors in immunocompetent hosts with relevant microenvironment
  • ✓ Implement castration protocol to model androgen-dependent and castration-resistant disease progression
  • ✓ Monitor bioluminescent and fluorescent tumor growth longitudinally in vivo
Protocol

Biopharma Insights The goal of this protocol is to demonstrate the intra-prostatic injection of prostate cancer cells, with subsequent castration. Orthotopic pre-clinical models of androgen-dependent and castration-resistant prostate cancer are critical to study the disease in the context of a clinically relevant tumor microenvironment and an immunocompetent host.

Difficulty
advanced
Total time
~45 min per mouse for injection; tumors assessable within 2–4 weeks; castration performed at specified timepoint; total study duration 4–8 weeks
Model organism
Mouse (syngeneic prostate cancer model, immunocompetent host)
Biosafety
BSL-2

Steps

1
Perform intra-prostatic injection of cancer cells

Inject bioluminescent and fluorescent prostate cancer cells directly into the murine prostate gland via surgical approach. This establishes an orthotopic tumor in its native anatomical microenvironment.

▶ 00:59
2
Analyze representative orthotopic tumor characteristics

Assess and document bioluminescent and fluorescent signal from established orthotopic prostate tumors, including tumor take rate, growth kinetics, and imaging modality validation.

▶ 04:37
3
Interpret androgen-dependent and castration-resistant model outcomes

Review protocol conclusions demonstrating how castration-induced androgen deprivation and subsequent disease progression are modeled in the orthotopic syngeneic system for preclinical efficacy testing.

▶ 06:17
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