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Pharmacology JoVE (Open Access) Citable · DOI

A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats

DOI: 10.3791/58384-v
What you'll learn
  • ✓ Implement a morphine conditioned place preference protocol in rats
  • ✓ Measure incubation of craving behavior over time
  • ✓ Analyze CPP memory induction and preference scores
  • ✓ Interpret craving-related behavioral changes in addiction models
Protocol

Here, a morphine conditioned place preference (CPP) protocol is described to measure incubation of craving in rats.

Difficulty
advanced
Total time
~2–4 weeks (includes conditioning, withdrawal, and testing phases)
Model organism
Rat (strain not specified)
Biosafety
BSL-1

Steps

1
Perform conditioned place preference procedure

Conduct the CPP protocol with morphine administration and environmental pairing in rats. Establish drug-context associations through repeated conditioning sessions.

▶ 00:48
2
Analyze representative CPP memory induction data

Quantify and interpret CPP memory formation by measuring preference scores and behavioral responses during test sessions, evaluating craving incubation.

▶ 02:34
3
Interpret results and experimental conclusions

Summarize findings on incubation of craving dynamics and discuss implications for addiction neurobiology research.

▶ 03:20
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