Home › Cell Biology › A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice
Cell Biology JoVE (Open Access) Citable · DOI

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

DOI: 10.3791/53731-v
What you'll learn
  • ✓ Prepare and execute anterior chamber microbead injection in mice
  • ✓ Position magnetic field to occlude iridocorneal angle and elevate intraocular pressure
  • ✓ Monitor and measure ocular hypertension-induced retinal ganglion cell loss
Protocol

Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.

Difficulty
advanced
Total time
~45 min per mouse for injection; IOP monitoring and assessment over 2–4 weeks
Model organism
Mouse (strain not specified; C57BL/6J assumed standard)
Biosafety
BSL-1

Steps

1
Prepare microneedle for anterior intracameral injection

Assemble and calibrate the microneedle apparatus for precise delivery of magnetic microbeads into the anterior chamber of the mouse eye.

▶ 01:02
2
Induce ocular hypertension via magnetic microbead occlusion

Inject magnetic microbeads into the anterior chamber and use an external magnet to attract them to the iridocorneal angle, blocking aqueous humour outflow and elevating intraocular pressure.

▶ 02:13
3
Assess ocular hypertension and retinal ganglion cell loss

Measure intraocular pressure and evaluate retinal ganglion cell survival to confirm glaucoma induction and characterize disease progression.

▶ 06:51
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