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Cell Biology JoVE (Open Access) Citable · DOI

A Model of Self-limited Acute Lung Injury by Unilateral Intra-bronchial Acid Instillation

DOI: 10.3791/60024-v
What you'll learn
  • ✓ Prepare and instill hydrochloric acid into mouse lung bronchus
  • ✓ Induce unilateral acute lung injury modeling aspiration-induced ARDS
  • ✓ Monitor and quantify self-limited pulmonary inflammation and recovery
  • ✓ Assess lung injury severity through histological and physiological endpoints
Protocol

Selective intra-bronchial acid instillation to the left lung in mice results in unilateral and self-limited acute lung injury that models human acute respiratory distress syndrome (ARDS) induced by gastric acid aspiration.

Difficulty
advanced
Total time
~45–60 min per mouse (surgical preparation, instillation, and immediate recovery monitoring)
Model organism
Mouse (strain not specified)
Biosafety
BSL-1

Steps

1
Prepare hydrochloric acid instillation solution

Prepare the required concentration of HCl for bronchial administration. This chapter covers solution preparation and standardization prior to surgical procedures.

▶ 00:53
2
Surgically prepare mouse for instillation

Anesthetize, intubate, and position the mouse for selective left-lung acid delivery. Includes securing airway access and preparing surgical field.

▶ 01:39
3
Perform selective intra-bronchial HCl instillation

Deliver measured HCl volume to the left main bronchus under direct visualization, inducing unilateral acute lung injury. Monitor for successful instillation.

▶ 03:46
4
Assess lung injury outcomes and recovery

Quantify acute inflammatory response, lung edema, and tissue damage over time. Evaluate self-limited nature of injury through histological and physiological measurements.

▶ 05:11
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