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Analytical Chem JoVE (Open Access) Citable · DOI

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

DOI: 10.3791/51893-v
What you'll learn
  • ✓ Design and plan tissue microarray construction for biomarker studies
  • ✓ Annotate digital pathology slides using virtual slide platforms
  • ✓ Execute automated tissue arraying and next-generation TMA construction
  • ✓ Optimize TMA quality control and data integration workflows
Protocol

The protocol aims at optimizing the construction and quality of tissue microarrays for biomarker research. It includes aspects of planning and design, digital pathology, virtual slide annotation, and automated tissue arraying.

Difficulty
advanced
Total time
~3–5 days (planning through construction and quality assessment)

Steps

1
Scan tissue slides for digital pathology

Digitize histological tissue sections using slide scanning technology to create high-resolution virtual slides suitable for annotation and analysis.

▶ 01:30
2
Annotate regions of interest on digital slides

Use virtual slide annotation tools to mark and classify tissue regions, defining areas for microarray spot selection and biomarker localization.

▶ 02:34
3
Construct tissue microarray using automated arraying

Employ automated tissue arraying systems to extract annotated tissue cores and assemble them into microarray blocks with precise spatial coordination.

▶ 04:14
4
Validate next-generation tissue microarray construction

Perform quality control and verification of completed ngTMA blocks to ensure tissue integrity, core placement accuracy, and suitability for downstream biomarker analysis.

▶ 08:19
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