Home › Cell Biology › A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
Cell Biology JoVE (Open Access) Citable · DOI

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

DOI: 10.3791/56415-v
What you'll learn
  • ✓ Set up and calibrate a laser speckle contrast imaging system for microcirculation measurement
  • ✓ Prepare participants and acquire speckle contrast images while controlling for movement artifact
  • ✓ Analyze alternating blood flow speeds in a single experimental session
Protocol

Biopharma Insights This study introduces a novel technique for the measurement and analysis of alternating speeds of blood microcirculation in a single experiment using a laser speckle contrast imager.

Difficulty
intermediate
Total time
~30–45 min per participant

Steps

1
Configure laser speckle contrast imaging system

Assemble and calibrate the moorFLPI LSCI device according to manufacturer specifications. Verify laser alignment and detector sensitivity before participant imaging.

▶ 01:51
2
Prepare participant for microcirculation imaging

Position participant, identify imaging site on skin, and stabilize the limb or region to minimize involuntary movement during speckle acquisition.

▶ 02:24
3
Acquire speckle contrast images during alternating flow states

Capture laser speckle images while participant performs controlled movements or speed changes (e.g., rest/exercise alternations) to record blood microcirculation at different velocities.

▶ 03:27
4
Measure and quantify blood flow from speckle data

Process acquired speckle images using moorFLPI software to extract perfusion maps and calculate microcirculatory flow values, accounting for movement artifact compensation.

▶ 05:36
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