Home › Pharmacology › A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System
Pharmacology JoVE (Open Access) Citable · DOI

A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

DOI: 10.3791/59201-v
What you'll learn
  • ✓ Construct and operate a mouse positioning device for intranasal delivery
  • ✓ Administer brain-targeting peptide-siRNA formulations intranasally with proper technique
  • ✓ Verify CNS gene silencing via fluorescent peptide and siRNA distribution
Protocol

Here, we present a protocol using a mouse positioning device that enables the appropriate placement of mice for the intranasal administration of a brain-targeting peptide-siRNA formulation enabling effective gene silencing in the central nervous system.

Difficulty
intermediate
Total time
~15–20 min per mouse (anesthesia induction, positioning, delivery, recovery)
Model organism
Mouse (strain not specified in abstract)
Biosafety
BSL-1

Steps

1
Position mouse using the delivery device

Place the anesthetized mouse in the positioning device to ensure optimal nasal alignment for intranasal administration. The device stabilizes head angle and prevents aspiration.

▶ 00:28
2
Administer peptide-siRNA via intranasal route

Deliver the brain-targeting peptide-siRNA formulation intranasally using the positioning device to enable direct CNS access. Maintain proper dosage and delivery rate.

▶ 01:23
3
Assess CNS distribution and gene silencing outcomes

Image fluorescently labeled peptide and siRNA to confirm successful distribution to the CNS and verify target gene silencing efficacy.

▶ 03:28
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