Home › Cell Biology › Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models
Cell Biology JoVE (Open Access) Citable · DOI

Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models

DOI: 10.3791/52319-v
What you'll learn
  • ✓ Adapt human VFSS protocols for murine swallowing assessment
  • ✓ Perform videofluoroscopic imaging and analysis in mice
  • ✓ Quantify dysphagia parameters from fluoroscopic swallow videos
  • ✓ Translate swallowing dysfunction findings to disease models
Protocol

This study successfully adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating translational dysphagia research.

Difficulty
advanced
Total time
~45–60 min per mouse (including imaging and analysis)
Model organism
Mouse (unspecified strain, disease models)
Biosafety
BSL-1

Steps

1
Prepare equipment and mouse positioning

Set up videofluoroscopic imaging apparatus, position mouse for swallow study, and ensure proper radiographic alignment for capturing swallow mechanics.

▶ 01:33
2
Perform videofluoroscopic swallow testing

Administer barium contrast and acquire real-time fluoroscopic video of the swallowing sequence, capturing oropharyngeal and esophageal phases.

▶ 03:07
3
Analyze videofluoroscopic swallow videos

Review recorded videos frame-by-frame to measure swallow kinematics, timing, and coordination metrics relevant to dysphagia assessment.

▶ 05:03
4
Quantify swallowing parameters and interpret results

Extract numerical values for videofluoroscopic swallow parameters such as swallow duration, penetration, and aspiration events to characterize dysphagia severity.

▶ 06:13
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