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Microbiology JoVE (Open Access) Citable · DOI

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Name: Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
Uploaded: 2026-10-13
Duration: 11 min
Description: Learn adenoviral Cre delivery for conditional gene knockout in primary mouse embryonic fibroblasts. This method achieves nearly 100% transgene efficiency to study Rac1 function in actin dynamics.

DOI: 10.3791/2160-v

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