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Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Name: Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
Uploaded: 2026-10-12
Duration: 11 min 28 s
Description: Learn affinity precipitation of active Rho-GEFs using GST-RhoA(G17A) from epithelial cell lysates. This method enables researchers to track GEF activation via Western blotting and densitometry for quantitative analysis.

DOI: 10.3791/3932-v

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